Lab Practical – Gel electrophoresis of albumins and globulins

Well, today was a lab day at University! Although I was rather shocked when I turned up in the lab and realised that it wasn’t the normal way we go in AND THEN the massive lab room was split in half? It looked like such a different alien world, not even kidding!!!

I had my partner in crime with me today as well, Sam! We don’t seem to have as many labs together lately which is such a shame as I feel we make a great team… when things work, which they have been doing lately!

Today’s practical was for “Introduction to Clinical Biochemistry” involving SDS-PAGE, gel electrophoresis of proteins, acrylamide gel… some very nasty chemicals and stuff, to put it simply. But what is a lab without a few hazards? And we were all very sensible and nothing bad happened.

Fig 1: Finally feeling more confident with my pipetting skills!
Fig 2: Mes amino (lab partner in crime) Sam, as well as fellow lab Intro to Clincical Biochem buddies, Ruari and Paige.

Actually, the lab went pretty well… and there was definitely some clear results too.

Fig 3: BioRad PAGE mini gel apparatus once set up and running.
Fig 4: Results. You can see the marker ladder which corresponds to different proteins, and faintly, our results (although my camera flash refused to work in such a bright lab)

We were given a normal sample, gamma globulin sample, bovine serum albumin (BSA) sample and then an unknown, of which we had to figure out. Sadly, I was quite useless when put on the spot to figure out what was different/going on with the unknown sample… besides it had a larger band than the normal sample. But you live and you learn, right? We did figure it out eventually thanks to wonderful lecturers being nice and patient and guiding with me and mes amino (Sam is my amigo, therefore a la Science, she is mes amino) and after that, everything made sense!

Lab 1 - dinner
Fig 5: Obligatory student dinner of a Big Mac and fries, with obligatory diet coke and a million tissues pulled out of the dispenser when you only want one…

And of course, after every lab, there is a trip to McDonalds, as standard.

And then the weary student returns home to a cat who won’t leave her alone. I have had to type around her tiny body to post this, so if there are spelling mistakes, its her fault because she loves me just THAT much!

Lab 1 - the cat
Fig 6: The infamous princess and ruiner of work, Princess Snooty Pants, Magnus Bane

I’m looking forward to the next lab, which according to my timetable is next Monday morning for “Biology of Human Disease”, a personal favourite module of mine!

 

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